Curated Optogenetic Publication Database

Search precisely and efficiently by using the advantage of the hand-assigned publication tags that allow you to search for papers involving a specific trait, e.g. a particular optogenetic switch or a host organism.

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1.

LILAC: enhanced actin imaging with an optogenetic Lifeact.

blue AsLOV2 Schneider 2
Nat Methods, 30 Jan 2023 DOI: 10.1038/s41592-022-01761-3 Link to full text
Abstract: Lifeact is a popular peptide-based label of actin filaments in live cells. We have designed an improved Lifeact variant, LILAC, that binds to actin in light using the LOV2 protein. Light control allows the user to modulate actin labeling, enabling image analysis that leverages modulation for an enhanced view of F-actin dynamics in cells. Furthermore, the tool reduces actin perturbations and cell sickness caused by Lifeact overexpression.
2.

LILAC: Enhanced actin imaging with an optogenetic Lifeact.

blue AsLOV2 Schneider 2
bioRxiv, 8 Apr 2022 DOI: 10.1101/2022.04.07.487416 Link to full text
Abstract: We have designed an improved Lifeact variant that binds to actin under the control of light using the LOV2 protein. Light control enables one to subtract the pre-illumination signal of the unbound label, yielding an enhanced view of F-actin dynamics in cells. Furthermore, the tool eliminates actin network perturbations and cell sickness caused by Lifeact overexpression.
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